Adrenomedullin2 Antagonist Alters Fetoplacental Growth Rate during Rat Late Gestation
نویسنده
چکیده
Adrenomedullin-2 (ADM2), a novel calcitonin/calcitonin-gene related peptide family with an exon-intron structure similar to that of ADM. The aim of the present study is to evaluate the role of Adrenomedullin-2 (ADM2) on fetoplacental development in female pregnant albino rats and to evaluate the effect of adrenomedullin2 antagonist (ADM21747) on fetal and placental development in late gestation of pregnant rats. The pregnant rats were continuously exposed with Adrenomedullin2 antagonist (ADM217-47) at the concentration of 50 μg and 200 μg / rat / day by inserting osmotic mini pumps from the 18 gestational day onwards and the rats were sacrificed on 22 gestational day to evaluate the placental and fetal growth. Earlier studies of antagonism of ADM2 function during rat pregnancy caused fetal growth restriction, and also decreased the fetoplacental weight. The histopathological studies ADM2 infusion caused impairment of labyrinths and distortion of deciduas in placenta. In immunohistochemical studies the presence of caspase-3 protein was observed in the uterus and placental tissues, this is confirmed by western blot analysis, the caspase-3 was significantly increased in both uterus and placental tissues were as Bcl-2 was significantly decreased when compared with the controls. Therefore the present study reveals the potential role of ADM2 in regulation of fetoplacental growth during rat late gestation.
منابع مشابه
In vivo monitoring of fetoplacental Vegfr2 gene activity in a murine pregnancy model using a Vegfr2-luc reporter gene and bioluminescent imaging
BACKGROUND Vascular endothelial growth factor receptor-2 (VEGFR2) plays a pivotal role in angiogenesis by eliciting vascular endothelial cell growth when bound to VEGF, a powerful pro-angiogenic ligand. While Vegf and Vegfr2 are expressed throughout gestation, the latter third of gestation in mice is characterized by a marked increase in fetoplacental angiogenesis. Thus, the objective of this s...
متن کاملExpansion of the fetoplacental vasculature in late gestation is strain dependent in mice
How the fetoplacental arterial tree grows and expands during late gestational development is largely unknown. In this study, we quantified changes in arterial branching in the fetal exchange region of the mouse placenta during late gestation, when capillarization increases rapidly. We studied two commonly used mouse strains, CD1 and C57Bl/6 (B6), at embryonic days (E)13.5, 15.5, and 17.5. B6 mi...
متن کاملApelin Controls Fetal and Neonatal Glucose Homeostasis and Is Altered by Maternal Undernutrition.
The adequate control of glucose homeostasis during both gestation and early postnatal life is crucial for the development of the fetoplacental unit and adaptive physiological responses at birth. Growing evidences indicate that apelin and its receptor, APJ, which are expressed across a wide range of tissues, exert important roles in glucose homeostasis in adults. However, little is known about t...
متن کاملPrenatal stress alters seizure thresholds and the development of kindled seizures in infant and adult rats.
Stressful events during gestation and in the neonatal period have important effects on the later physical and mental health of the offspring. The present study tested the hypothesis that pre- and/or postnatal stress would affect seizure susceptibility in infant and adult rats, using the hippocampal kindling model. Prenatal stress consisted of daily restraint of the dam under bright light (for 4...
متن کاملEndothelial NO synthase augments fetoplacental blood flow, placental vascularization, and fetal growth in mice.
It is not known whether eNOS deficiency in the mother or the conceptus (ie, placenta and fetus) causes fetal growth restriction in mice lacking the endothelial NO synthase gene (eNOS knockout [KO]). We hypothesized that eNOS sustains fetal growth by maintaining low fetoplacental vascular tone and promoting fetoplacental vascularity and that this is a conceptus effect and is independent of mater...
متن کامل